:QUESTIONSTART:
:SHORTNAME: APB 5.2.1c
:QUESTIONTYPE:MC
:QUESTIONTEXT: What takes place during the study of genomics?
:ANSWER: How nucleotide sequences are read and regulated and variations thereof:VAL:100
:ANSWER: How characteristics are transmitted from parents to offspring:VAL:0
:ANSWER: How diseases affect organisms and interact with cells:VAL:0
:ANSWER: How genetic information is transferred from one organism to another:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.3.3a
:QUESTIONTYPE:MC
:QUESTIONTEXT: Why is marker-assisted selection such a valuable technology for livestock producers?
:ANSWER: Markers give visually conclusive, quantitative data for producers:VAL:100
:ANSWER: Markers will express themselves within a single generation of offspring:VAL:0
:ANSWER: Markers are only visible in the phenotype of a live animal:VAL:0
:ANSWER: Markers are used to develop expected progeny differences over many generations:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.4.1a
:QUESTIONTYPE:MC
:QUESTIONTEXT: What is the process of using plants to convert waste into a less hazardous form called?
:ANSWER: Phytoremediation:VAL:100
:ANSWER: Bioremediation:VAL:0
:ANSWER: Conservation:VAL:0
:ANSWER: Biodefense:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.4.2a
:QUESTIONTYPE:MATCH
:QUESTIONTEXT: Match the biofuel source to the correct product.
:ANSWER: Corn and sugarcane
:MATCH: Bioethanol
:ANSWER: Corn stover and switchgrass
:MATCH: Cellulosic ethanol
:ANSWER: Vegetable oil and animal fats
:MATCH: Biodiesel
:ANSWER: Biodigestors and wastes
:MATCH: Biomethanol
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.4.3b
:QUESTIONTYPE:MC
:QUESTIONTEXT: Which fuel source is a product of fermentation?
:ANSWER: Ethanol:VAL:100
:ANSWER: Biodiesel:VAL:0
:ANSWER: Methanol:VAL:0
:ANSWER: Natural gas:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.4.4a
:QUESTIONTYPE:MC
:QUESTIONTEXT: Which of the following is defined as “guiding a cautious approach to scientific research to avoid pursuing potentially harmful situations?”
:ANSWER: The precautionary principles:VAL:100
:ANSWER: Bioremediation:VAL:0
:ANSWER: Esterification:VAL:0
:ANSWER: Ethical considerations of biotechnology:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.1.1c
:QUESTIONTYPE:MC
:QUESTIONTEXT: What is the study or manipulation of living things?
:ANSWER: Biotechnology:VAL:100
:ANSWER: Bioremediation:VAL:0
:ANSWER: Domestication:VAL:0
:ANSWER: Fermentation:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.1.2a
:QUESTIONTYPE:MA
:QUESTIONTEXT: Which of the following practices are utilized in biotechnology today? (Select all that apply.)
:ANSWER: Molecular biology:VAL:100
:ANSWER: Bioengineering:VAL:100
:ANSWER: Bioinformatics:VAL:100
:ANSWER: Bioremediation:VAL:100
:ANSWER: Electrical engineering:VAL:0
:ANSWER: Anabolism:VAL:0
:ANSWER: Cation exchange:VAL:0
:ANSWER: Germination:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.1.3a
:QUESTIONTYPE:MC
:QUESTIONTEXT: Treating an animal to prevent disease uses which biotechnology innovation?
:ANSWER: Vaccine:VAL:100
:ANSWER: Stem cells:VAL:0
:ANSWER: Genetic engineering:VAL:0
:ANSWER: Fermentation:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.1.4a
:QUESTIONTYPE:MC
:QUESTIONTEXT: Why is it important to ask the ethical questions when deciding whether or not to adopt new technology?
:ANSWER: Ethical questions generate discussions that drive policy and regulation:VAL:100
:ANSWER: Ethical questions halt all research until approval has been given:VAL:0
:ANSWER: Ethical questions only affect researchers in alboratories:VAL:0
:ANSWER: Ethical questions ignore personal opinions and are based solely on scientific thinking:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.2.1b
:QUESTIONTYPE:MC
:QUESTIONTEXT: How do you know the specific laboratory safety procedures and rules?
:ANSWER: You should receive training before any lab work begins:VAL:100
:ANSWER: Every laboratory has the same procedures and rules:VAL:0
:ANSWER: You can develop your own:VAL:0
:ANSWER: Lab technicians are expected to know all specifics before taking a position:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.2.2d
:QUESTIONTYPE:MC
:QUESTIONTEXT: Where should chemicals be stored in a laboratory?
:ANSWER: In a chemical cabinet:VAL:100
:ANSWER: In a fume hood:VAL:0
:ANSWER: In an autoclave:VAL:0
:ANSWER: On the lab counter:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.2.4c
:QUESTIONTYPE:MC
:QUESTIONTEXT: Why are Good Laboratory Practices critical in biotechnology?
:ANSWER: To ensure the quality and integrity of laboratory data:VAL:100
:ANSWER: To ensure the quality and safety of manufactured products:VAL:0
:ANSWER: To ensure all processes lead to patents:VAL:0
:ANSWER: To ensure safety and protection of employees in a workplace:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.2.5b
:QUESTIONTYPE:MC
:QUESTIONTEXT: What should the display read on a 2-20 micropipet set to 3 mmicroliters?
:ANSWER: 03.0:VAL:100
:ANSWER: 00.3:VAL:0
:ANSWER: 00.03:VAL:0
:ANSWER: 30.0:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.3.1a
:QUESTIONTYPE:TF
:QUESTIONTEXT: “Sterile technique” refers to a set of protocol used to prevent microbial contamination in an environment.
:ANSWER: True:VAL:100
:ANSWER: False:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.3.2d
:QUESTIONTYPE:MC
:QUESTIONTEXT: Which of the following fits the description of a eukaryotic cell?
:ANSWER: Genetic material is contained within a membrane-enclosed nucleus:VAL:100
:ANSWER: Genetic material is contained within a single molecule floating in the cytoplasm:VAL:0
:ANSWER: The smallest of cells:VAL:0
:ANSWER: Typically unicellular in orgnaization:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.3.3a
:QUESTIONTYPE:MC
:QUESTIONTEXT: How many hydrogen bonds join adenine and thymine together?
:ANSWER: 1:VAL:0
:ANSWER: 2:VAL:100
:ANSWER: 3:VAL:0
:ANSWER: 4:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 1.3.4a
:QUESTIONTYPE:MC
:QUESTIONTEXT: What is a genotype?
:ANSWER: The genetic makeup of an organism:VAL:100
:ANSWER: It prescribes for a specific phenotype:VAL:0
:ANSWER: The physical expression of a genetic trait:VAL:0
:ANSWER: The percent of a population with an expressed trait:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 2.1.1a
:QUESTIONTYPE:MC
:QUESTIONTEXT: Which fields of study are combined in molecular biology?
:ANSWER: Genetics and biochemistry:VAL:100
:ANSWER: Nutrition and ecology:VAL:0
:ANSWER: Genetics and calculus:VAL:0
:ANSWER: Chemistry and biology:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 2.1.2a
:QUESTIONTYPE:MC
:QUESTIONTEXT: Which of the following is a chemical extraction method used to extract DNA from a cell?
:ANSWER: Cell lysis solution:VAL:100
:ANSWER: Freezing the cell:VAL:0
:ANSWER: Mashing the cell:VAL:0
:ANSWER: Transcription:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 2.1.3b
:QUESTIONTYPE:MC
:QUESTIONTEXT: What is a restriction enzyme?
:ANSWER: An enzyme that cleaves a DNA molecule at a recognized restriction site:VAL:100
:ANSWER: An enzyme that codes for “stop” in DNA transcription at a recognized restriction site:VAL:0
:ANSWER: An enzyme that joins Okazaki fragments in the leading strand at a recognized restriction site:VAL:0
:ANSWER: An enzyme that begins to unwind the parental double helix at the Methionine code:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 2.2.2d
:QUESTIONTYPE:MA
:QUESTIONTEXT: When transforming bacteria, such as E. Coli HB 101, to have ampicillin resistance and green fluorescence which of the following plates are used as controls in the experiment? Select all that apply.
:ANSWER: LG agar with -pGLO:VAL:100
:ANSWER: LB agar/amp with -pGLO:VAL:100
:ANSWER: LB agar/amp/ara with +GLO:VAL:0
:ANSWER: LB agar/amp with +pGLO:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 2.2.2f
:QUESTIONTYPE:MC
:QUESTIONTEXT: How is the success of bacterial genetic engineering measured?
:ANSWER: Transformation efficiency:VAL:100
:ANSWER: Acquired immune response:VAL:0
:ANSWER: Bacterial quantification:VAL:0
:ANSWER: Biuret test:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 2.2.3d
:QUESTIONTYPE:MC
:QUESTIONTEXT: If a protein needs to be purified for pharmaceutical or food application, which method is more appropriate?
:ANSWER: High level purity:VAL:100
:ANSWER: Crude extract:VAL:0
:ANSWER: Bioinformatics:VAL:0
:ANSWER: Tissue culture:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 3.1.1c
:QUESTIONTYPE:MC
:QUESTIONTEXT: What are proteins composed of?
:ANSWER: Amino acids:VAL:100
:ANSWER: Restriction enzymes:VAL:0
:ANSWER: Ligases:VAL:0
:ANSWER: DNA polymerase:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 3.1.2d
:QUESTIONTYPE:MC
:QUESTIONTEXT: What is the process of converting mRNA into amino acids known as?
:ANSWER: Transcription:VAL:0
:ANSWER: Translation:VAL:100
:ANSWER: Restriction:VAL:0
:ANSWER: Replication:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 3.1.3a
:QUESTIONTYPE:MATCH
:QUESTIONTEXT: Match the following types of assays to a situation in which it might be useful.
:ANSWER: The more accurate test for casein protein in milk using a spectrophotometer.
:MATCH: Bradford
:ANSWER: Using a protein reagent to measure protein molecules in food using peptide bond concentration.
:MATCH: Biuret
:ANSWER: Testing for genetically modified organisms (GMOs) using antibodies to test for antigens.
:MATCH: Enzyme-linked immunoabsorbent assay (ELISA)
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 3.1.4a
:QUESTIONTYPE:MC
:QUESTIONTEXT: Which of the following is an example of an innate immune response?
:ANSWER: Circulating macrophages and killer cells destroy all foreign bodies:VAL:100
:ANSWER: A foreign body elicits the production of antibodies:VAL:0
:ANSWER: A specific response is activated by initial contact with a foreign body:VAL:0
:ANSWER: DNA within cells mutates to repel foreign bodies:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.1.1b
:QUESTIONTYPE:MA
:QUESTIONTEXT: Which of the following are common genetic modifications to crops? Select all that apply.
:ANSWER: Herbicide resistance, such as RoundUp tolerance:VAL:100
:ANSWER: Pest resistance, such as Bt avoidance:VAL:100
:ANSWER: Multiple crops per plant, such as tomato/potato plants:VAL:0
:ANSWER: Temperature resistance, such as growth during frost:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.1.2a
:QUESTIONTYPE:MC
:QUESTIONTEXT: How is polymerase chain reaction (PCR) used to detect genetic modification?
:ANSWER: PCR generates multiple copies of the protein of interest:VAL:100
:ANSWER: PCR generates Taq polymerase:VAL:0
:ANSWER: PCR generates DNA polymerase:VAL:0
:ANSWER: PCR generates a positive control:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.1.3b
:QUESTIONTYPE:MC
:QUESTIONTEXT: Why are genetically modified plants so controversial?
:ANSWER: Many people feel GMOs are “unnatural”:VAL:100
:ANSWER: Many people feel GMOs are too expensive:VAL:0
:ANSWER: Many researchers have proved that GMOs do not offer benefits:VAL:0
:ANSWER: Many researchers have proved that immediate dangers are posed by GMOs:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.2.2b
:QUESTIONTYPE:MC
:QUESTIONTEXT: What is an explant?
:ANSWER: A simple piece of plant tissue which gives rise to a new plant by developing new tissues from a cellular level:VAL:100
:ANSWER: A complex piece of plant which gives rise to a new plant by developing missing tissues:VAL:0
:ANSWER: A new plant propagated by modified root and stem structures:VAL:0
:ANSWER: The culture or medium required to complete micropropagation for plants:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.2.4a
:QUESTIONTYPE:MC
:QUESTIONTEXT: What enzyme is used to cut DNA?
:ANSWER: Restriction enzyme:VAL:100
:ANSWER: Helicase:VAL:0
:ANSWER: Primase:VAL:0
:ANSWER: Polymerase:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 4.3.1c
:QUESTIONTYPE:MC
:QUESTIONTEXT: Following the introduction of a new disease in an animal’s body, what response should a researcher find?
:ANSWER: Antibodies:VAL:100
:ANSWER: Antigens:VAL:0
:ANSWER: Macrophages:VAL:0
:ANSWER: White blood cells:VAL:0
:QUESTIONEND:

:SHORTNAME: APB 5.1.1b
:QUESTIONTYPE:MC
:QUESTIONTEXT: Why are valid experiments comprised of many trials repeating the same version of an experiment?
:ANSWER: To compare variations of the same treatment:VAL:100
:ANSWER: To ensure all-or-nothing results:VAL:0
:ANSWER: To gather multiple sets of contradictory data:VAL:0
:ANSWER: To utilize simple trial and error to solve a problem:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 5.1.2a
:QUESTIONTYPE:MC
:QUESTIONTEXT: If a researcher wanted to learn more about the best procedure for a biotechnology application, what should he or she do first?
:ANSWER: Literature review:VAL:100
:ANSWER: Publishing research in a journal:VAL:0
:ANSWER: Conducting experiments:VAL:0
:ANSWER: Developing a hypothesis:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 5.1.3a
:QUESTIONTYPE:MC
:QUESTIONTEXT: How does a researcher ensure that a treatment group results in differences?
:ANSWER: By establishing a control group to compare to:VAL:100
:ANSWER: By establishing multiple treatment groups:VAL:0
:ANSWER: By conducting the experiment one time:VAL:0
:ANSWER: By creating diverse treatments for each trial:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 5.2.1c
:QUESTIONTYPE:MC
:QUESTIONTEXT: What takes place during the study of genomics?
:ANSWER: How nucleotide sequences are read and regulated and variations thereof:VAL:100
:ANSWER: How characteristics are transmitted from parents to offspring:VAL:0
:ANSWER: How diseases affect organisms and interact with cells:VAL:0
:ANSWER: How genetic information is transferred from one organism to another:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 2.2.1a
:QUESTIONTYPE:MC
:QUESTIONTEXT: What is a plasmid?
:ANSWER: A circular DNA molecule a few thousand base pairs in length:VAL:100
:ANSWER: A single-celled prokaryotic microorganism:VAL:0
:ANSWER: A bacterial virus:VAL:0
:ANSWER: DNA and protein in a coiled, rod-shaped form:VAL:0
:QUESTIONEND:

:QUESTIONSTART:
:SHORTNAME: APB 2.2.2b
:QUESTIONTYPE:MC
:QUESTIONTEXT: Which of the following methods are used to facilitate transformation?
:ANSWER: Electroporation, heat shock, and chemicals:VAL:100
:ANSWER: Cell lysis, purification, and transcription:VAL:0
:ANSWER: Electrophoresis, polymerase chain reaction, and extraction:VAL:0
:ANSWER: Restriction enzymes, agarose, and bacteriophage:VAL:0
:QUESTIONEND: